PfuTurbo Cx Hotstart DNA Polymerase Boosts Your BS-Seq PerformanceOctober 19, 2010Amplifying converted DNA is one of the trickiest steps in any Bisulfite conversion based protocol. A mistake here could stop your study cold if the amplification fails, or worse yet, you might be introducing errors that you won’t see until you’ve spent hours combing through useless sequencing data. Pfu-Turbo Cx Hotstart DNA Polymerase from Agilent’s Stratagene […]
Comparative Epigenomics Branches OutOctober 19, 2010When you’re comparing epigenomes, it’s easy to miss the forest for the trees. Because the epigenetic “fruit” on each nucleotide can vary with factors like cell type, differentiation state, and the environment, it’s often tricky to spot patterns on the epigenome-wide scale. In a recent Nature Biotechnology commentary, Aleksandar Milosavlijevic of the NIH Epigenomics Roadmap […]
How to Tame the MethylomeOctober 18, 2010So many ways to analyze genomic DNA methylation, so little time (and money). With so many choices available these days it’s not easy to figure out the best method to use for methylome studies. A new survey of two Nature Biotechnology papers by Stephan Beck from the UK’s University College London puts together the vital […]
miRNA Sponges Making a Big SplashOctober 15, 2010A few years back synthetic miRNA decoys, nicknamed “miRNA sponges”, were developed as an innovative new way to inhibit miRNA activity by bumping up the number of miRNA targets to “soak up” a specific miRNA. Now recent reports are popping up showing evidence that naturally occurring miRNA sponges have been around all along, we just […]
miRNAs Host New Variety ShowOctober 5, 2010If variety is the spice of life, then miRNAs are a bowl of 3-alarm Texas chili. A new study in the journal RNA used NextGen sequencing techniques to reveal that miRNAs show quite a bit of sequence variety, even within individual miRNA species. The research, conducted by Lik Wee Lee and colleagues at the Institute […]