DNA Methylation qPCR Panels Keep Stem Cells in CheckMay 8, 2012Every day, in labs all over the world, stem cells are changing. Sure they may lack (lineage) commitment, but these ultra-promising cellular blank canvases aren’t living sedentary epigenetic lifestyles. DNA methylation in stem cells is on the move with age and passages and you might not even know it. Sequencing still isn’t quite feasible for […]
oxBS-Seq Breaks Through 5hmC Sequencing BarrierApril 26, 20125-Hydroxymethylcytosine (5hmC) has always been a bear to sequence, mostly because it’s virtually indistinguishable from its close relative, 5-methylcytosine (5mC) using classic bisulfite sequencing techniques. Now a new sample preparation method has hit the scene that can discriminate between cytosine, 5mC and 5hmC at single base resolution, called oxidative bisulfite sequencing (oxBS-Seq) that essentially smashes […]
Sequencing DNA Methylation Past, Present, Future with Dr. Wolf ReikApril 26, 2012Dr. Wolf Reik discusses the challenges in DNA methylation sequencing and the next big swings in epigenetic modification sequencing and discovery. This interview took place at the Keystone Symposia’s Epigenomics and Chromatin Dynamics joint meeting in January, 2012. This video was brought to you by epigenetics supporters at EMD Millipore. Sequencing DNA Methylation […]
Argonaute AGO2 Gives miRNAs a Little Trim Around the EdgesApril 17, 2012Isn’t there always one person in a group of friends who’s a little weird or different? It turns out that AGO2, is a little different from its Argonaute paralogs, AGO1, 3, and 4. Researchers in Singapore are reporting that unlike other Argonautes, oddball AGO2 trims mammalian miRNAs, changing their functions during development. The team knew […]
miRNAs Decide Which Honey Bees Do Which Honey-Do’sApril 17, 2012It seems nobody has been busier than the honey bee lately when it comes to being a model epigenetic organism. (Watch your back c. elegans!) Over the last year or so, we’ve seen how HDAC inhibitors affect the bee caste system, as well as how social cues alter DNA methylation patterns in the brains of […]
