Keeping Up with Live Cell DNA LoopingJune 25, 2013The reality TV era has made voyeurs of us all. So it makes sense that we’d want to take a peek inside live cells too. Now comes a report of a new method that shows, for the first time, DNA getting loopy inside live E. coli cells. Johns Hopkins University Med School researchers say that […]
DeepSAGE Digs up Expression Differences in 3’ EndsJune 24, 2013It isn’t always a good thing to get the end of something, but researchers say that by grabbing onto 3’ ends of transcripts and tagging them, DeepSAGE digs deep, getting low-level transcripts and getting new info on their “back-ends.” That could be important for figuring out exactly how gene expression changes with disease. “It was […]
HDAC-Glo™ Brightens up HDAC Activity ScreeningJune 21, 2013Histone Deactylase (HDAC) researchers have been clamoring for more sensitive and reliable HDAC assays in order to generate the high quality data that previous techniques don’t always deliver. The HDAC-Glo™ I/II Assays and Screening Systems from Promega aim to shine new light on the matter with highly sensitive assays that ditch the old, troublesome false […]
RIP-Seq Shows miRNA Expression and Function Not Always a MatchJune 20, 2013There is a saying that, “each microRNA can have hundreds of target mRNAs, and each mRNA can be targeted by hundreds of miRNAs”. If that’s true, then how the heck are we supposed to know what’s going on in that crazy transcriptome? What does it mean when a miRNA and predicted target don’t explain the […]
Application Note: Automated Bisulfite ConversionJune 14, 2013Unlike more mainstream assays, bisulfite conversion approaches have been dependent on manual manipulation of spin plates and columns or have been of limited throughput. By adapting the clean-up of bisulfite converted DNA to a magnetic bead based procedure and coupling it to the Freedom EVO® platform, Zymo Research has opened the door to high-throughput bisulfite […]
